TABLE 1.
Primers used in this study
| Name | Sequence | Additional information |
|---|---|---|
| H1_fw | AATGGTCCCAAAATTGTC | Colony PCR for segregation at slr0168 insertion and sequencing |
| H2_rv | CTGTGGGTAGTAAACTGGC | Colony PCR for segregation at slr0168 insertion and sequencing |
| KanF_back_Seq | TCCCGTTGAATATGGCTC | Colony PCR for insertion at slr0168 and sequencing |
| 1556_Nde_F | AATCATATGAAAATCGCTTTTTTTAGCAG | slr1556 ORF amplification |
| 1556_Bam_R | AATGGATCCTTAATGGGGACAGATTACTTGGT | slr1556 ORF amplification |
| 1556_out_fw | AATTTGGGGTGAAGCTGGGT | Colony PCR for slr1556 deletion |
| 1556_out_rv | CCGTTAATACTATCGTTGCTTCATTT | Colony PCR for slr1556 deletion |
| slr1556_800bp_fw | AAGGTTGACCAAGGAATGGTTGGTT | slr1556 knockout plasmid |
| slr1556_800bp_rv | GTTTACGAATGGACTGATTCCACCA | slr1556 knockout plasmid |
| slr1556_EcorI_fw | GAATTCATGAAAATCGCTTTTTTTAGC | Slr1556 overexpression for purification |
| slr1556_XhoI_rev | CTCGAGTTAATGGGGACAGATTACTTGG | Slr1556 overexpression for purification |