TABLE 3.
Characterization of the kinetic properties of purified, recombinant Slr1556a
| Compound | Measured directionality | KH (mM) | Vmax (μmol/mg/min) | Hill coefficient |
|---|---|---|---|---|
| Pyruvate | Pyruvate reductase | 0.39 ± 0.17 | 236.12 ± 13.12 | 1.97 ± 0.47 |
| Hydroxypyruvate | Hydroxypyruvate reductase | 6.7 ± 1.4 | 78.14 ± 8.42 | 1.38 ± 0.24 |
| Glyoxylate | Glyoxylate reductase | 2.43 ± 0.01 | 175.52 ± 12.08 | 2.34 ± 0.02 |
| d-Lactic acid | Lactate dehydrogenase | 7.93 ± 0.22 | 10.65 ± 0.82 | 2.52 ± 0.29 |
a
The kinetic parameters for the Hill equation were calculated in SigmaPlot 11.0 as described by Goutelle et al. (31), assuming a baseline response. Data are mean values of results (n = 2).