Figure 5. NEP transcript knockdown in BEAS-2B cells with NEP-specific siRNA.
Cells (at 3000 cells/cm2) were transfected with control siRNA (middle column) or NEP siRNA (right column). Cells were harvested 30 hours post-transfection, and the effect of siRNA transfection was determined by Western blotting. NEP protein expression levels were normalized to [3-actin (*P < 0.05).