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. Author manuscript; available in PMC: 2016 Dec 21.
Published in final edited form as: FEBS Lett. 2015 Nov 26;589(24 0 0):3959–3968. doi: 10.1016/j.febslet.2015.11.037

Fig. 5.

Fig. 5

Age-dependent accumulation of ubiquitylated substrates and diubiquitin, and 20S proteasome activities, between Ranbp2−/−::Tg-Ranbp2CLDm-HA and wild type mice exposed to light-stress. In comparison to wild type mice, Ranbp2−/−::Tg-Ranbp2CLDm-HA mice present decreased and increased levels of ubiquitylated substrates and diubiquitin, respectively, at 8 weeks of age (A, C), but their levels are similar between genotypes at 24 weeks of age (B, C). (D) Retinal extracts of 24 week-old Ranbp2−/−::Tg-Ranbp2CLDm-HA and wild type mice have similar chymotrypsin and trypsin-like activities of the 20S proteasome, but Ranbp2−/−::Tg-Ranbp2CLDm-HA have modestly lower caspase-like activity than wild type mice. Data shown represent the mean ± S.D., n=4–6. Legend: +/+, wild type; −/−::Tg-Ranbp2CLDm-HA, Ranbp2−/−::Tg-Ranbp2CLDm-HA; di-Ub, diubiquitin; poly-UbP, poly-ubiquitylated proteins; hsc70, heat shock cytosolic protein 70; n.s., non-significant.