Figure 1.

(A) SEC elugram of AMCA-PLGA; the black line indicates the refractive index of the PLGA expressed as relative units (RIU), and the gray line indicates the ultraviolet absorption by AMCA at 350 nm expressed as relative milli-units (mAU). (B) Stability studies via fluorescence intensity measurements of AMCA-PLGA in DMSO for >120 hours at 4°C (solid line, crosses), 20°C (dotted line, triangles) and 37°C (broken line, circles). Data are expressed as ratios of I/I₀ and shown as the mean ± SD of three independent experiments.

Abbreviations: AMCA-PLGA, 7-amino-4-methyl-3-coumarinylacetic acid-poly(d,l-lactide-co-glycolide); DMSO, dimethyl sulfoxide; mAU, milli-absorption unit; PLGA, poly(d,l-lactide-co-glycolide); RIU, refractive index unit; SD, standard deviation; SEC, size exclusion chromatography.