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. 2016 Feb 5;11:575–583. doi: 10.2147/IJN.S98401

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© 2016 Klinger-Strobel et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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CLSM images of Burkholderia cepacia (A, C, E, G, I) and Staphylococcus aureus (B, D, F, H, J) biofilms.

Notes: The images represent biofilm sections located ~2 μm above the glass surface. The biofilms were treated for 4 hours with PBS (A and B), AMCA-PLGA MP (C and D), AMCA-PLGA NP (E and F), Chi-AMCA-PLGA MP (G and H), and Chi-AMCA-PLGA NP (I and J). Viable cells are visualized in green; dead cells are visualized in red; and MP and NP are visualized in blue. The scale bar on image J also applies to images A–H.

Abbreviations: AMCA-PLGA, 7-amino-4-methyl-3-coumarinylacetic acid-poly(d,l-lactide-co-glycolide); Chi, chitosan; CLSM, confocal laser scanning microscopy; MP, microparticles; NP, nanoparticles; PBS, phosphate buffered saline.