Table 1. KD,app and koff measurements for FANA and DNA aptamers.
| 1FANA aptamers and various DNA aptamers | 2KD,app values (pM) | 2koff (s−1) |
|---|---|---|
| FANA aptamers | ||
| FANA random pool | 6100±700 | |
| FA1 | 4±3 | 0.00011±0.00005 |
| FA2 | 16±6 | |
| FA3 | 270±20 | |
| FA1Δ1–14 | 5±1 | 0.00009±0.00002 |
| FA1GCstems | 12±5 | 0.00013±0.00006 |
| FA1GCstemsΔL4/S4 | 1500±400 | |
| FA1GCstemsΔL2 | 260±40 | |
| FA1GCstemsΔL1 | 18±6 | |
| FA1GCstemsL1→stem | 19±5 | |
| MuLV (random pool)a | 2200±400 | |
| MuLV (FA1)a | 1300±400 | |
| AMV (random pool)a | 3200±300 | |
| AMV (FA1)a | 5000±2100 | |
| DNA aptamers | ||
| 38 NT SELEX | 160±20 | |
| 38NT2,4-methyl | 14±2 | 0.00010±0.00004 |
| R1T G-quadruplex | 6±1 | 0.00014±0.00003 |
| FA1→DNAa | 23 000±9000 | |
| Random pool DNAa | 24 000±4000 | |
| RNA aptamer | ||
| 26 nt RNApseudoknot | 210±10 | |
1—Refer to text and Figure 4 for information on the various FANA aptamers. All experiments were with HIV-1 RT except those designated as MuLV and AMV.
2—KD,app and dissociation rate constant (koff) measurements shown in this table were performed using nitrocellulose filter bind as described in Materials and Methods. Results are an average of three or more independent experiment ± standard deviations.
aDetermined using 100 pM alternative KD assay (see Material and Methods).