Table 1.

List of primers. qRT-PCR primers were designed to span an exon boundary in order to prevent interaction with genomic DNA as opposed to cDNA. Tpcn2 flox primers flank a loxP site, producing differential product sizes for floxed and wild type alleles. Excision event primers flank both loxP sites allowing for confirmation of the Cre excision event.

qRT-PCR	Tpcn2	F-ATGATGAAGAAGACCCTGAAGTG R-TGTGCTTCATCCTTCTCACC
	β-actin	F-CGAGTCGCGTCCACCC R-CATCCATGGCGAACTGGTG
Genotyping	Tpcn2 flox	F-ATTCTGGGCTGCTACTGTGG R-GTTGGTCCTCCTAGGCTGTG
	Ins1cre	F-ATGTCCAATTTACTGACCG R-CGCCGCATAACCAGTGAAAC
Excision event		F-CTGCTACTGTGGGTGGTATATGG R-CAATGTTATTTGAACTGATGGCGAG