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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Drug Metab Pharmacokinet. 2015 May 7;30(4):269–275. doi: 10.1016/j.dmpk.2015.04.004

Table 1.

Sequence of primers for amplification of cDNA.

Isoforms Primer Sequence Annealing temperature (°C)
UGT1A1 Forwarda 5′-CCT TGC CTC AGA ATT CCT TC-3′ 60
Reverse 5′-CAC AGG ACT GTC TGA GGG ATT TT-3′
UGT1A3 Forward 5′-GAG AGG TGT CAG TGG TGG ATA TTC T-3′ 60
Reverse 5′-CAC AGG ACT GTC TGA GGG ATT TT-3′
UGT1A4 Forwardb 5′-CCT GCT GTG TTT TTT TGG AGG T-3′ 60
Reversea 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′
UGT1A5 Forwarda 5′-ACA ATA TGT CTT TGA TCA TA-3′ 60
Reversea 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′
UGT1A6 Forwardb 5′-CAA CTG TAA GAA GAG GAA AGA C-3′ 54
Reversea 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′
UGT1A7 Forwardb 5′-CCC CTA TTT TTT CAA AAATGT CTT-3′ 60
Reversea 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′
UGT1A8 Forwarda 5′-GGT CTT CGC CAG GGG AAT AG-3′ 60
Reversea 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′
UGT1A9 Forwarda 5′-GAA CAT TTA TTA TGC CAC CG-3′ 60
Reversea 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′
UGT1A10 Forwarda 5′-CTC TTT CCT ATG TCC CCA ATG A-3′ 60
Reversea 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′
Ugt2b1 Forward 5′-TTG GGC CGA GCA ATG AAT CT-3′ 60
Reverse 5′-GAT AGC AGC TCA CCA CAG GG-3′
Ugt2b5 Forward 5′-TTA TGC GCC ACA AAA GAG CC-3′ 60
Reverse 5′- AAT CAA GTC AAG TGT TTA GAA GGT G -3′
Ugt2b34 Forward 5′-TTC CAG TGG TTG GCA TTC CT-3′ 60
Reverse 5′-ACA CGA AGA TGC TTG GCT CC-3′
Ugt2b35 Forward 5′-CTT GTA CAG AGG GGC CAT GAA-3′ 60
Reverse 5′- TGG GAC CTC AAA AGT CCA TTC -3′
Ugt2b36 Forward 5′- AAG GTG TTG GTA TGG CCG GT-3′ 66
Reverse 5′-AGT CCA TCT TTC CAC AGC CTT-3′
Ugt2b37 Forward 5′-ACA GCT TTT GAA TGT GTG GAC T-3′ 60
Reverse 5′-GCG ATT AGC TCC CCA CCA AA-3′
Cyp2b10 Forward 5′-GCA AGC CAT GTT GCT CCT AA-3 60
Reverse 5′-CTT GGA GCC CTG GAG ATT TGG-3′
CPHc Forward 5′-CAG ACG CCA CTG TCG CTT T-3′ 60
Reverse 5′-TGT CTT TGG AAC TTT GTC TGC AA-3′
a

Nakamura et al. (2008) [45].

b

Izukawa et al. (2009) [19].

c

Fujiwara et al. (2012) [46].