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. 2016 Feb 12;11(2):e0149571. doi: 10.1371/journal.pone.0149571

Fig 4. Genetic and physical interactions of Dbp5 and ribosomal export factors.

Fig 4

(A) Dbp5 genetically interacts with the established ribosomal transport factors Nmd3 and Mtr2. The indicated strains were spotted in serial dilutions onto plates either selecting for a covering the respective wild type gene containing plasmid (selective media) or for the loss of this plasmid (FOA = 5-Fluoroorotic Acid). The strains rat8-3 and rat8-2 in combination with nmd3-2 or mtr2-33, which has a specific export defect for ribosomal subunits, show synthetic growth defects after three days at 30°C. (B) Physical interactions of Dbp5 with Nmd3, Xpo1 and Rio2. Western blot analyses show co-precipitated Nmd3-myc in the Dbp5-GFP immunoprecipitation (left panel) and co-precipitated Dbp5 in the Xpo1-GFP and Rio2-GFP pull-downs (right panel). The DBP5-GFP expressing strain and the wild type (WT) were transformed with an NMD3-13xmyc containing plasmid. Detection of Hem15 served as a negative control and of Rps3 as a positive control.