Fig 8. Dbp5 interacts with Mex67 in vivo and in vitro.

(A) Dbp5 interacts mainly RNA-dependently with Mex67 in vivo. Western blot analyses of GFP-Dbp5 immunoprecipitations show strong co-precipitation of Mex67 without RNase A treatment, while addition of RNase A leads to a weaker, but reproducible interaction with Mex67. Detection of Hem15 served as non-binding control. (B) Direct interaction of recombinant GST-Dbp5 and Mex67, which is increased by ATP addition. Western blot analyses of Glutathione Sepharose pull-downs with GST-Dbp5 or GST and the purified heterodimer His-Mtr2 and Mex67 are shown in the presence or absence of 1 mM ATP and 0.2 mg/ml RNase A. The corresponding Coomassie-stained gel is depicted in S8D Fig.