Fig 4. Anti-fibrotic effect of Prdx5 in TGF-β induced renal fibrosis.

NRK49F cells were transiently transfected with various amounts of Wild-type Prdx5. After one day, cells were starved with DMEM/low glucose media with 0.5% FBS for one day, and then exposed to TGF-β for three days. Changes in expression of fibrotic marker proteins by Prdx5 over-expression were measured (A). Bar graphs show mean Prdx5/β-actin (B), α-SMA/β-actin (C), and fibronectin/β-actin expression (D) as measured by densitometry. *p < 0.05, TGF-β treated Mock vs. untreated Mock; ^‡ p < 0.05, TGF-β treated Mock vs. WT Prdx5