Fig 6. Effects of NF-κB inhibition on rHMGB-1-induced HESC proliferation and TLR4 expression.

The NF-κB pathways was blocked using TPCA-1, and rHMGB-1-dependent cell proliferation was measured (A). TLR4 expression was analyzed using qRT-PCR (B) and western blotting (C, D) following treatment with different concentrations of rHMGB-1. Changes in IL-6 secretion were measured following inhibition of the NF-κB pathway by TPCA-1 (E). *, P < 0.05 versus the control.