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. 2016 Feb 12;11(2):e0149388. doi: 10.1371/journal.pone.0149388

Fig 2. Rapamycin treatment promotes expression of transglutaminase 2.

Fig 2

(A) Comparison of Tgm2 transcript levels in Tsc2-/- and Tsc1-/- MEF cells treated with either vehicle or rapamycin (n = 3). (B) Immunoblots of TGM2 and phospho-S6 in Tsc2-/- and Tsc1-/- MEF cells treated with either vehicle or rapamycin. (C) Tgm2 transcript levels in rapamycin-treated MCF-7 cells at various time points (n = 3). (D) Immunoblots of TGM2 and phospho-S6 in vehicle- and rapamycin-treated MCF-7 cells at various time points. (E) Tgm2 transcript levels in rapamycin-treated 786-O cells at various time points (n = 3). (F) Immunoblots of TGM2 and phospho-S6 in vehicle- and rapamycin-treated 786-O cells at various points. (G) Immunoblots of TGM2 and phosphor-S6 in in vehicle- and rapamycin-treated Tsc1-/- and Tsc2-/- MEFs, MCF-7 and 786-O cells transfected with vehicle or TGM2 plasmids. All data are shown as means ± S.D. ** P < 0.01, *P < 0.05, Student’s t-test.