Fig 4. Effect of miR-21 on capillary network formation of HUVECs.

(A) After transfection with miR-21 mimics, miR-21 mimic negative control, antagomir-21 or antagomir-21 negative control, HUVECs were exposed to OGD and then trypsinized and seeded onto Matrigel. Cells were incubated and allowed to form networks for 12 h, then stained with calcein-AM (Green) for 15 min. Bar = 200 μm. (B) Comparison of the number of branch nodes in the treatment groups relative to respective negative control groups at 12 h. Values represent means±S.D; n = 3; *p<0.05.