Table 3. Analytical sensitivity of HtLAMP-Pv, compared with other published P. vivax LAMP primers, using a DNA dilution series of clinical sample with qPCR confirmed parasitemia.

Each sample was tested in duplicate in the HtLAMP platform using each of the three P. vivax LAMP primer sets. The dilution at which both duplicates were positive was the limit of detection for each of the primer sets in the HtLAMP platform (Pos* indicates dilutions at which only one of the duplicates was positive). HtLAMP-Pv was able to detect 1.4 parasites/ μL compared with previously published P. vivax LAMP primers.

Sample	Quantitative PCR (parasites/μL calculated)	[23] 18s rRNA target	[30] Pvr64 target	HtLAMP-Pv cox1 target
1 Original	90,000	Pos	Pos	Pos
2	45,000	Pos	Pos	Pos
3	22,500	Pos	Pos	Pos
4	11,250	Pos	Pos	Pos
5	5625	Pos	Pos	Pos
6	2813	Pos	Pos	Pos
7	1406	Pos	Pos	Pos
8	703	Pos	Pos	Pos
9	352	Pos*	Pos	Pos
10	176		Pos	Pos
11	88			Pos
12	44			Pos
13	22			Pos
14	11			Pos
15	5.5			Pos
16	2.7		Pos*	Pos
17	1.4			Pos
18	0.7			Pos*
19	0.3