Table 1. Target, primer sequences, target sizes, and components of PCR.

Target (PCR amplicon sizes)	PCR components
Human KCNE1 (45 bp)	10 μL 2x PrimeSTAR Max mix or iTaq Universal SYBR PCR mix
	1 μL For primer (5’ CCC ATT CAA CGT CTA CAT CGA GTC 3’)
	1 μL Rev primer (5’ TCC TTC TCT TGC CAG GCA T 3’)
	2 μL SpeedSTAR HS polymerase
	4 μL nuclease-free water
	2 μL template
	Total volume 20 μL (use 10 μL per reaction)
Human DNA (600 bp, 500 bp, 400 bp, 300 bp, 200 bp, 100 bp)	7.5 μL Isohelix DNA Quality Check Kit human primer mix
	12.5 μL Isohelix DNA Quality Check Kit amplification mix
	2.5 μL nuclease-free water
	2.5 μL human genomic DNA
	Total volume 25 μL
Chlamydia trachomatis 16S rRNA gene (165 bp)	10 μL 2x Premix Ex Taq
	1 μL For primer (5’ TAG GCG GAT TGA GAG ATT GG 3’)
	1 μL Rev primer (5’ TAT TCC CAA GCG AAA GTG CT 3’)
	0.5 μL Probe (5’ AGA ATC TTT CGC AAT GGA CG 3’)
	5.5 μL nuclease-free water
	2 μL template
	Total volume 20 μL
HIV gag (~85 bp)	10 μL 2x One Step RT-PCR buffer III
	0.5 μL PrimeScript RT enzyme mix II
	0.5 μLTakara Ex Taq HS
	1 μL For primer (5’ GGC TAC ACT AGA AGA AAT GAT GAC AGC AT 3’)
	1 μL Rev primer (5’ GCT CAT TGC TTC AGC CAA AAC TCT TGC 3’)
	0.5 μL Probe (5’ AGT AGG AGG ACC CGG CCA TA 3’)
	2.5 μL nuclease-free water
	4 μL template (at various concentrations)
	Total volume 20 μL
Ebola virus NP (112 bp)	10 μL 2x One Step RT-PCR buffer III
	0.5 μL PrimeScript RT enzyme mix II
	0.5 μLTakara Ex Taq HS
	1 μL For primer (5’ AGG GTG ATC CAA CAA CCT TAA T 3’)
	1 μL Rev primer (5’ CTC TGG GTG TTC AGG TTC AAA 3’)
	0.5 μL Probe (5’ TGC TTG TTT GAC TGT GAA CTA ATG CTG TC 3’)
	2.5 μL nuclease-free water
	4 μL template
	Total volume 20 μL
Dengue virus I (112 bp)	12.5 μL 2x One Step RT-PCR buffer III
	0.5 μL PrimeScript RT enzyme mix II
	0.5 μLTakara Ex Taq HS
	0.5 μL For primer (5’ CAA AAG GAA GTC GYG CAA TA 3’)
	0.5 μL Rev primer (5’ CTG AGT GAA TTC TCT CTG CTR AAC 3’)
	0.45 μL Probe (5’ CAT GTG GYT GGG AGC RCG C 3’)
	8.05 μL nuclease-free water
	2 μL template
	Total volume 25 μL