Fig 5. Combination antibody treatment caused little change in effector or memory CD4⁺ T cells in the liver.

Mice infected with a low (5 x 10⁶) parasite inoculum, and treated with mAb alone or a combination of anti-GITR mAb on day 14 p.i., and anti-IL-10R mAb on days 14, 19 and 24 p.i., as indicated. Rat IgG was used as a control. A. Hepatic CD4⁺ T cells were analysed by FACS by first gating on CD4⁺ TCRβ⁺ populations. Activated CD4⁺ T cells were selected based on CD44 and CD62L expression, and then further divided based on KLRG-1 and CD127 expression. B. Effector CD4⁺ T cells were defined as KLRG-1⁺ CD127^-, C. central memory T (T_CM) CD4⁺ T cells were defined as KLRG-1^- CD127⁺, and D. effector memory T (T_EM) CD4⁺ T cells were defined as KLRG-1^- CD127^-) frequency and number are shown (n = 5 mice per group, one independent experiment from 1–3 independent experiments).