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. 2016 Feb 12;11(2):e0149000. doi: 10.1371/journal.pone.0149000

Fig 1. Schematic of experimental protocols.

Fig 1

A. For phosphoprotein studies hearts were perfused with Krebs Henseleit buffer at 37°C in Langendorff setups. The perfused hearts were allowed to stabilize for 5 min before perfusion for 15 min without (Ctrl p) or with rhCCN2 (CCN2 p; 250 nM). B and C. For the ischemia-reperfusion studies Langendorff-perfused hearts were allowed to stabilize for 25 min before start of no-flow global ischemia. No-flow ischemia was maintained for 40 min (Ctrl 40i, CCN2 40i, Ctrl 40i +LY and CCN2 40i +LY) or 25 min (Ctrl 25i and CCN2 25i). In the CCN2 40i, CCN2 40i + LY and CCN2 25i the first 15 min of the 60 min reperfusion were in the presence of 250nM recombinant human CCN2. In the Ctrl 40i + LY and CCN2 40i + LY the entire perfusion was performed in the presence of 50μM LY294002.