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. 2016 Feb 12;11(2):e0149000. doi: 10.1371/journal.pone.0149000

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© 2016 Kaasbøll et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Western blot analysis of phospho-GSK3β (Ser-9) contents in extracts of murine hearts following ex vivo perfusion of the hearts (Langendorff-perfusion) in the absence or presence of increasing concentrations of recombinant human CCN2. Each lane on the Western blot represents tissue from individual hearts. The histogram demonstrates group means of phospho-GSK3β contents assessed by densitometric analysis of the immunoreactive bands on the Western blot. The phospho-GSK3β (Ser-9) contents are displayed relative to total immunoreactive GSK3β contents. (Each group is mean of 2–3 ex vivo-perfused hearts)