Table 1. 16S rRNA gene-based identification of soil isolates and population analysis of 16S rRNA gene for five cellobiosan-utilizing bacterial isolates and four levoglucosan-utilizing bacterial isolates based on sequences of Iowa COBS soil microbial community.
Isolates S1, S2, S3, S4, S5, and F6 utilize cellobiosan. Isolates 1, 2, 3 and 4 utilization levogluocsan. OTU: Operational taxonomic unit, here is defined as genes sharing 97% sequence similarity in the COBS dataset. Abundance: the relative abundance of OTU within COBS dataset.
| NCBI BLAST (nr) | COBS | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Closest Match | Identity (%) | Length of match | Gene ID | Identity (%) | Length of match | Abundance | OTU | Relative abundance | |
| S1 | Enterobacter sp SJZ-6 | 99 | 604/605 | dbj|LC014955.1| | 99 | 127/128 | 360 | 922761 | 0.0110 |
| S2 | Sphingobacterium multivorum | 97 | 1316/1359 | dbj|AB680844.1| | 99 | 252/253 | 50 | 891031 | 0.0015 |
| S3 | Acinetobacter oleivorans JC3-1 | 98 | 1352/1385 | gb|KM983423.1| | 99 | 251/253 | 70 | 889025 | 0.0021 |
| S4 | Microbacterium sp FXJ8.207 | 98 | 1353/1383 | gb|KM507662.1| | 98 | 270/277 | 10 | 1045797 | 0.0003 |
| S5 | Microbacterium sp FXJ8.203 | 98 | 1302/1330 | gb|JQ012996.1| | 98 | 269/277 | 10 | 1045797 | 0.0003 |
| F6 | Cryptococcus sp | 95 | 686/722 | gb|KM587000.1| | N/A | N/A | N/A | N/A | N/A |
| 1 | Bacillus horikoshii | 97 | 1361/1396 | gb|KJ534599.1| | 100 | 253/253 | 22 | 591482 | 0.0037 |
| 2 | Bacillus korlensis 1 | 95 | 1336/1402 | gb|KC443095.1| | 98 | 244/249 | 158 | 42013 | 0.38 |
| 3 | Bacillus korlensis 2 | 96 | 1344/1407 | gb|KC443095.1| | 94 | 236/250 | 158 | 42013 | 0.38 |
| 4 | Bacillus sp 5138 | 97 | 1358/1400 | gb|KC236668.1| | 98 | 250/254 | 302 | 848816 | 0.036 |