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. 2016 Feb 12;11(2):e0149064. doi: 10.1371/journal.pone.0149064

Fig 4. Effect of the E1E2p7 mutations on E1E2 glycoprotein expression.

Fig 4

HuH-7-RFP-NLS-IPS cells were transfected with WT or mutated HCV genomes. An assembly-deficient virus (ΔE1E2) was used as control. Expression of E1E2 viral glycoproteins and actin was analyzed in cell lysates 72h post-electroporation by western blotting with specific MAbs (A4 [anti-E1], 3/11 [anti-E2] and C4 [anti-actin]).