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Integrated area of the TrpCN fluorescence spectrum of 2WCNP versus the mole fraction of DMSO (χDMSO) in the H2O-DMSO binary solvent. These data have been normalized such that the value obtained in pure DMSO is 1.0. The smooth line is the best fit of the data to a modified Hill equation (i.e., Eq. 3) with the following parameters: Imin = 0.11 ± 0.02, Imax = 1.10 ± 0.01, k = 0.18 ± 0.01, n = 3.20 ± 0.29, a = −0.30 ± .01, and b = 1.20 ± 0.01.
