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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Hypertension. 2016 Jan 18;67(3):630–639. doi: 10.1161/HYPERTENSIONAHA.115.06946

Figure 4.

Figure 4

Increased markers of hypoxia in fetuses from pregnant rats with elevated T. Pimonidazole (A) and hypoxia-inducible factor (HIF) 1α (B) immunoblotting were used to identify hypoxia in the placenta at gestation day 20 in control and T fetuses. Representative Western blots for Pimonidazole, HIF1α, and β-actin are shown at top; blot density obtained from densitometric scanning of Pimonidazole and HIF1α normalized to β-actin is shown at bottom. Values are given as means ± SEM of 6 fetuses in each group. *P≤.05 vs respective control. #P≤.05 vs T female fetueses.