Figure 4. Anaerobic storage and glycolysis.

The central panel shows a schematic representation of the oxygen-dependent metabolic modulation mechanism. Deoxyhemoglobin under anaerobic conditions would bind to the N-terminal cytosolic domain of band 3. This would in turn results in the release of glycolytic enzymes from cdB3, increased levels of glycolysis, and deactivation of the pentose phosphate pathway (PPP OFF).

Select metabolomics data for the three arms are shown in the side panels. These results are consistent with the model suggesting the total anaerobiosis promotes faster glucose consumption and accumulation of glycolytic intermediates, DPG, and end products than routine storage under normoxia (control). On the other hand, anaerobic storage with 5% CO₂ supplementation (AN[CO₂]) promotes ATP preservation at the expense of DPG. Solid line=AN, Dotted line=AN[CO₂], Dashed line=Control