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Yeast strains expressing Cdc11-TAP and Cdc11-GFP (metabolically labelled with 13C6,15N4-L-arginine and 13C6,15N2-L-lysine) are blocked with the indicated methods and subjected separately to affinity purification. The eluates are mixed and subjected to separation by SDS-PAGE followed by nano HPLC-ESI-MS/MS analysis and statistical data evaluation.
