Table 2. Validated target genes for S. japonicum miRNAs.
| miRNAs | Target gene IDs | Name of target genes | MiRNA:mRNA duplex* | Mfe (kcal/mol) | Validated # |
|---|---|---|---|---|---|
| Bantam | AY223092.1 | Serine-arginine repressor | Target 5`U CUUU A U 3` | -22.5 | By miRNA suppression |
| GGCU AUC CGAUCUCG | |||||
| UCGA UAG GCUAGAGU | |||||
| miRNA 3`AAU C 5` | |||||
| FN323394.1 | FUS-interacting serine-arginine-rich protein 1 | Target 5`G UU A U 3` | -22.6 | By luciferase assay/ miRNA suppression | |
| GGUUUU AUC CGAUCUCG | |||||
| UCGAAA UAG GCUAGAGU | |||||
| miRNA 3`U C 5` | |||||
| AY815078.1 | Smad1 | Target 5`U UG C 3` | -19.9 | By miRNA suppression | |
| GGC UUGAUCGUG CUUA | |||||
| UCG AAUUAGCGC GAGU | |||||
| miRNA 3`A UA 5` | |||||
| Let-7 | FN314191.1 | Ribosomal protein S6 kinase 2 | Target 5`A C U A 3` | -28.6 | By luciferase assay |
| CAU CAAC GAACUACCUC | |||||
| GUG GUUG CUUGAUGGAG | |||||
| miRNA 3`UG U G5` | |||||
| miR-2a | FN321618.1 | Plasminogen activator inhibitor 1 | Target 5`A AG CUGAAU C3` | -24.7 | By luciferase assay |
| GUUCA GA UUGGCUGUG | |||||
| CAAGU UU GACCGACAC | |||||
| miRNA 3`G AG AU U5` | |||||
| miR-31 | EU370927 | Frizz7 | Target 5`G G G 3` | -22.0 | By luciferase assay |
| UCGUCGUGGUU UUGU | |||||
| AGCGGCAUUAG AACG | |||||
| miRNA 3`UCGA GU5` | |||||
| FN319623.1 | O-glycosyltransferase | Target 5`U C 3` | -28 | By miRNA suppression | |
| GCUUU UUGUAAUCUUGCC | |||||
| CGAAG GGCAUUAGAACGG | |||||
| miRNA 3`U C U5` | |||||
| miR-1989 | FN317226 | Asparagine-rich protein | Target 5`A U AUA UA U 3` | -26.4 | By luciferase assay |
| C AAGA GUGA ACACAGUUGA | |||||
| G UUCU UACU UGUGUCGACU | |||||
| miRNA 3`A C G | |||||
| miR-8 | FN313640 | Integral membrane protein GPR177 | Target 5`U AC UUAUUU G3` | -22.3 | By luciferase assay |
| GCAUUUU UACCUA AUAGUA | |||||
| CGUAGAA AUGGAU UGUCAU | |||||
| miRNA 3`C AAU5` | |||||
| DQ643829.2 | Wnt | Target 5`C A 3` | -20.8 | By luciferase assay | |
| GGCAUC ACC GC GUAUUA | |||||
| CCGUAG UGG UG CAUAAU | |||||
| miRNA 3`AAA AU U 5` | |||||
| miR-3479 | FJ753578.1 | Transforming growth factor receptor II | Target 5`A U ACUUA U3` | -20.3 | By luciferase assay |
| CGA GC UAAGUGCAAUA | |||||
| GUU CG AUUCACGUUAU | |||||
| miRNA 3`C CUUCC 5` |
*miRNA:mRNA pair analysis was performed using RNAhybrid (http://bibiserv.techfak.uni-bielefeld.de/rnahybrid/)
#luciferase assay = miRNA mimics down-regulate target mRNA sequences in mammalian cells; miRNA suppression = transfection of antisense miRNA sequences into schistosomes leads to increases in target mRNAs