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. 2016 Feb 12;11(2):e0148927. doi: 10.1371/journal.pone.0148927

Fig 9. Effects of hyperthermia on low and high dose EPI- and LPS-stimulated IL-6 regulation in isolated soleus muscle.

Fig 9

A) Effects of 1 h hyperthermia on IL-6 mRNA in low (1.2 ng/ml) and high dose (1 μg/ml) epinephrine (EPI). Each soleus measurement paired by animal, from one leg at 35°C; from the other leg treated for 1 h at 41°and then 1 h at 35°C. B. IL-6 protein secreted into the bath with exposure to low EPI, samples pulled and measured at 1 and 2 h after EPI and compared to fresh buffer (time 0). C) Identical to ‘B’ except high dose EPI. Bars represent paired comparisons. D) IL-6 mRNA response to low and high doses of LPS. E) IL-6 protein secretion in response to low dose LPS. F) IL-6 secretion in response to high dose LPS. n = 6 for all groups, except low dose mRNA to EPI (n = 5). # = P < 0.05 for paired measurements Wilcoxon signed ranks for paired samples. * = P < 0.05 for unpaired samples, Wilcoxon signed ranks for significance from lower limit threshold of IL-6 detection.