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. 2016 Feb 12;11(2):e0149180. doi: 10.1371/journal.pone.0149180

Fig 6. Effect of vitamin D on E-cadherin expression in lung epithelium of PBS and HRA-sensitized and challenged mice.

Fig 6

(A, B) Immunofluorescence image shows immunostaining of E-cadherin in the lungs of vitamin D-sufficient PBS control mice compared to vitamin D-sufficient HRA-sensitized and challenged mice (40× magnification). Right panels: Sections stained with rabbit anti-E-cadherin antibody and goat anti-rabbit Alexa Fluor 549 as secondary antibody; Left panels: Merged Alexa Fluor 549 and DAPI used to stain the nuclei; (C, D) Expression of E-cadherin in the lungs of vitamin D-supplemented PBS control mice compared to vitamin D-supplemented HRA-sensitized and challenged mice (40x magnification); (E) Corrected total fluorescence (CTF) of proteins in the airways was measured in arbitrary units (AU) using ImageJ software. The results are presented as mean ± SEM of 7 mice per group with 10 measurements per mouse, *p <0.05, ****p <0.0001.