Table 3. Characteristics of differentially methylated regions with at least 50% difference in methylation percentage, the same directional change and shared between and specific to the affected twin sisters.
Boxes 1 and 2: Methylation percentages of CpG islands as analyzed in replicates in 2 discordant monozygotic twins sisters. Box 3: Localization and associated genes (if known) of the 12 regions potentially associated with cardiovascular risk in adult women following pregnancies complicated by pre-eclampsia and/or HELLP.
| BOX1: Twin pair A | ||||
| # | Non-affected Value 1 | Affected Value 2 | Difference Q-value | Change % up/down |
| 1 | 17.111921 | 5.3906255 | 8.28E-07 | -68.50 |
| 2 | 24.686842 | 8.1035590 | 5.42E-06 | -67.17 |
| 3 | 49.505867 | 24.039217 | 6.76E-05 | -50.44 |
| 4 | 25.962824 | 39.875996 | 0.002803379 | 53.59 |
| 5 | 15.781162 | 24.678215 | 1.66E-05 | 56.38 |
| 6 | 47.121210 | 75.406510 | 0.001247889 | 60.03 |
| 7 | 33.518060 | 57.517000 | 1.30E-04 | 71.60 |
| 8 | 14.864865 | 26.935987 | 0.001854299 | 81.21 |
| 9 | 14.107142 | 27.698416 | 6.69E-09 | 96.34 |
| 10 | 15.517400 | 36.594770 | 0.003385719 | 135.83 |
| 11 | 3.3827498 | 13.666667 | 0.001085497 | 304.01 |
| 12 | 1.1363636 | 23.245615 | 0.003799252 | 1945.61 |
| BOX2: Twin pair B | ||||
| # | Non-affected Value 1 | Affected Value 2 | Difference Q-value | Change % up/down |
| 1 | 12.3949585 | 1.3513515 | 1.10E-06 | -89.10 |
| 2 | 14.4585260 | 2.6777172 | 3.35E-05 | -81.48 |
| 3 | 63.4640540 | 29.532772 | 1.27E-11 | -53.47 |
| 4 | 12.5000000 | 30.833334 | 0.005804231 | 146.67 |
| 5 | 16.9000000 | 30.226072 | 0.002090683 | 78.85 |
| 6 | 24.7163140 | 44.550762 | 2.70E-04 | 80.25 |
| 7 | 39.7959180 | 73.840880 | 1.20E-07 | 85.55 |
| 8 | 2.25225230 | 27.405165 | 1.34E-06 | 1116.79 |
| 9 | 13.8461550 | 24.765600 | 0.002160636 | 78.86 |
| 10 | 17.0431160 | 28.679487 | 0.002496304 | 68.28 |
| 11 | 5.9829063 | 30.860060 | 1.01E-06 | 415.80 |
| 12 | 25.0000000 | 71.428570 | 2.86E-04 | 185.71 |
| BOX3: Significantly changed DMRs in twin sisters at risk for CVD | ||||
| # | Chr(hg19) | Start | End | Feature |
| 1 | 1 | 180,922,916 | 180,923,692 | AK056657 |
| 2 | 1 | 41,981,789 | 41,982,416 | HIVEP3 |
| 3 | 2 | 8,596,908 | 8,597,573 | |
| 4 | 5 | 140,186,793 | 140,187,268 | PCDHA1 |
| 5 | X | 123,093,924 | 123,094,582 | STAG2 |
| 6 | 16 | 28,3798,131 | 28,379,974 | NPIPL1 |
| 7 | X | 153,046,342 | 153,046,768 | SRPK3 |
| 8 | 1 | 151,226,666 | 151,227,238 | PSMD4 |
| 9 | 4 | 2,048,730 | 2,049,144 | C4orf48 |
| 10 | 22 | 29,866,327 | 29,866,832 | AB020652 |
| 11 | 17 | 40,259,522 | 40,259,977 | DHX58 |
| 12 | 14 | 106,145,949 | 106,146,803 | IGHE |