Fig 7. Real-Time quantitative PCR of transiently transfected plasmid DNA purified 2 weeks post-transfection of HeLa S3 and SH-SY5Y cells.

HeLa S3 and SH-SY5Y cells were transiently transfected with pRc/CMV, natural CMV, SCP2 or SCP3 vector expressing EGFP, and harvested 14 days post-transfection (P.T.). Plasmid DNA was purified from cells and subjected to qPCR analysis with primers for the GAPDH, EGFP and Neomycin genes. Data shown are the averaged Ct values of 3 independent experiments (each performed in triplicates). Error bars represent SEM.