Table 3. Indicator species analysis (ISA) of the remaining 32 OTUs that were not previously assigned to one of the four individual cohorts.
Groups were designated as “Health” for healthy volunteers (combining the two groups with and without prior antibiotic exposure, and as “Disease” for subjects with CDI (combining the metronidazole and vancomycin groups). A representative sequence accession number from Silva (release 108) database is shown for all uncultured species as a reference OTU. Indicator value of species j in group k is the product of the percent relative abundance of each organism to a specific cohort along with its percent relative frequency. Those species that are significant after 100,000 Monte-Carlo randomizations of ecological communities are listed below.
| Indicator Value (%) | Relative Abundance (%) | Relative Frequency (%) | ||||||
|---|---|---|---|---|---|---|---|---|
| Max Group | Lowest Taxonomic Unit* | refOTU** | No Disease | Disease | No Disease | Disease | No Disease | Disease |
| No Disease | Faecalibacterium u2263 | JF220917 | 75.7 | 0.6 | 96.5 | 3.5 | 78.4 | 16.1 |
| Faecalibacterium u47 | BAAU01000008 | 75.3 | 0.1 | 98.5 | 1.5 | 76.5 | 9.7 | |
| Eubacterium eligens ATCC 27750 | 66.7 | 2.8 | 75.6 | 24.4 | 88.2 | 11.3 | ||
| Catenibacterium u1038 | DQ809643 | 55.1 | 0.1 | 96.9 | 3.1 | 56.9 | 1.6 | |
| butyrate producing bacterium L1 81 | 54.9 | 1 | 93.3 | 6.7 | 58.8 | 14.5 | ||
| Faecalibacterium u2080 | FJ509845 | 54.8 | 1.9 | 79.9 | 20.1 | 68.6 | 9.7 | |
| Faecalibacterium u979 | DQ808727 | 49.7 | 3.1 | 72.4 | 27.6 | 68.6 | 11.3 | |
| Faecalibacterium u487 | GQ898622 | 45.5 | 1 | 89.2 | 10.8 | 51 | 9.7 | |
| Ruminococcaceae u86 | AB506402 | 38.9 | 6.1 | 70.9 | 29.1 | 54.9 | 21 | |
| Subdoligranulum u1398 | DQ802754 | 38 | 0 | 97 | 3 | 39.2 | 1.6 | |
| Lachnospiraceae u397 | GQ897082 | 37.3 | 0 | 100 | 0 | 37.3 | 0 | |
| Roseburia u1603 | FJ363547 | 35.6 | 3.1 | 78.8 | 21.2 | 45.1 | 14.5 | |
| Prevotella stercorea | 35.1 | 0 | 99.6 | 0.4 | 35.3 | 1.6 | ||
| Lachnospiraceae u.349 | GQ898687 | 34.4 | 2.4 | 70.1 | 29.9 | 49 | 8.1 | |
| Lachnospiraceae u. 244 | EF403824 | 33.3 | 0 | 99.8 | 0.2 | 33.3 | 1.6 | |
| Ruminococcus u.1471 | DQ807522 | 30.8 | 0 | 98.3 | 1.7 | 31.4 | 1.6 | |
| Ruminococcaceae u.414 | EF403999 | 29.4 | 0 | 100 | 0 | 29.4 | 0 | |
| Lachnospiraceae u.40 | DQ905337 | 27.2 | 0.3 | 81.5 | 18.5 | 33.3 | 1.6 | |
| Alistipes u.906 | DQ805782 | 27.0 | 1.1 | 86.2 | 13.8 | 31.4 | 8.1 | |
| Disease | None | -- | -- | -- | -- | -- | -- | |
| None | Bacteroides uniformis | 37.0 | 23.5 | 43.9 | 56.1 | 84.3 | 41.9 | |
| Alistipes finegoldii | 33.8 | 9.8 | 59.4 | 40.6 | 56.9 | 24.2 | ||
| Parabacteroides merdae | 33.2 | 21 | 45.8 | 54.2 | 72.5 | 38.7 | ||
| Blautia u2062 | FJ366849 | 29.9 | 8.2 | 49.2 | 50.8 | 60.8 | 16.1 | |
| Alistipes u301 | EF402337 | 28.8 | 15 | 42 | 58 | 68.6 | 25.8 | |
| Ruminococcaceae u2003 | EU766380 | 25.6 | 4.5 | 76.8 | 23.2 | 33.3 | 19.4 | |
| Ruminococcaceae u940 | FJ370833 | 24.6 | 12.8 | 50.3 | 49.7 | 49 | 25.8 | |
| Barnesiella intestinihominis | 19.8 | 3.9 | 38.9 | 61.1 | 51 | 6.5 | ||
| Ruminococcaceae u277 | EF401722 | 16.1 | 12.3 | 45.7 | 54.3 | 35.3 | 22.6 | |
| Odoribacter splanchnicus | 14.5 | 18.2 | 29.6 | 70.4 | 49 | 25.8 | ||
| Faecalibacterium u.531 | FJ682262 | 9.2 | 9.3 | 17.4 | 82.6 | 52.9 | 11.3 | |
| Ruminococcaceae u1568 | FJ365212 | 9.1 | 6.5 | 42.4 | 57.6 | 21.6 | 11.3 | |
* u = uncultured followed by OTU number from the Silva database
** Accession number based on Silva (release 108)