Polymorphisms (locations) | Primer sequences | Restriction enzyme | SNP sequence | DNA fragment size (bp) |
---|---|---|---|---|
rs4898 |
F: 5’-TTCAGTCTATCAGAAGGCCG-3’ R: 5’-TAGCAAGAGAGATCAGGGAC-3’ |
T C |
Direct sequencing | |
rs6609533 |
F: 5’-CCTCTGGCTATTCTGTGTCC-3’ R: 5’-AGAGCAACAAGCAGATGTGC-3’ |
Bcl I |
G A |
312 bp 175 + 137 bp |
rs2070584 |
F: 5’-GTTGCTGATGACCTGGTGTG-3’ R: 5’-TGAGGATGAGGACAGTAACA-3’ |
T G |
Direct sequencing |
*F and R indicate forward and reverse primers, respectively.