Fig. 5.

Comparison of the inhibitory effects of menthol on the currents of wild-type (WT) hTRPV1 and hTRPV1 mutants (Y511A, S512Y, T550I) expressed in HEK293T cells. a–d Representative traces of the first heat-activated currents (upper) in the absence and presence of menthol (5 mM) with temperature changes (lower) in HEK293T cells expressing WT (a), Y511A (b), S512Y (c), and T550I (d) hTRPV1. Current–voltage relationships (right) in the absence and presence of menthol at the points indicated by asterisk in the left traces. e, f Comparison of the current densities activated by the first heat stimulation in the absence or presence of menthol (5 mM) in HEK293T cells at −60 mV (e) and +100 mV (f). **p < 0.01, *p < 0.05. n = 6–13. Data are shown as the mean ± SEM