FIG 1 .

Characterization of dequenching particles. (A) Coverslip-bound VSV pseudotyped with EBOV GPΔMuc displays high incorporation of mNG-P and GP, as indicated by KZ52-AF555 binding. Individual channels are pseudocolored white for greater visibility. DiD labeling was carried out so that the DiD signal remained visible when self-quenched. (B) Dequenching is detected as a sharp increase in DiD signal. An example of hemifusion is seen here. Bar, 10 µm. (C) Intensity trace for the indicated particle. (D) Representative traces for fast/specific (solid line) and slow/nonspecific (dotted line) dequenching. Rates were derived by fitting the traces to a one-phase nonlinear regression model. The plateau for the slow trace begins approximately 28 min after the onset of the signal increase. a.u., arbitrary units.