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External oleate causes a decrease in hilA expression independent of FadD, FadR, or the β-oxidation pathway; FadL is required for downregulation. Strains containing a hilA-lac fusion and the indicated mutations were grown under SPI1-inducing conditions in the absence or presence of 0.3 mM oleate. Tergitol NP-40 (0.2%) was present in all samples. β-Galactosidase activity is reported as mean ± standard deviation, where n is 4. Strains used were JS749 and JS2057 to -62. wt, wild type.
