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. 2015 Mar 3;87(2):130–137. doi: 10.1136/jnnp-2014-309932

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Gene expression kinetics of the Tumour necrosis factor-Related Apoptosis Inducing Ligand (TRAIL) and TRAIL receptors splicing variants in Jurkat cells on in vitro stimulation with interferon β (IFNβ). (A) TRAIL splicing variant genes are upregulated during 1-4 h of IFNβ treatment and decline thereafter. (B) Death receptor isoforms begin to upregulate at 8 h and reach a maximum at 24 h. (C) Decoy receptor isoforms begin to be induced at the 12 h time point and beyond. Expression levels are represented as relative expression compared with the reference gene GAPDH, using the ΔΔCt method. Mean and SD of each time point for each gene isoform is represented.