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. 2014 Dec 24;65(2):202–213. doi: 10.1136/gutjnl-2014-307949

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Characterisation of human gastric spheroids. (A) Confocal micrographs of human gastric spheroids (cross sections), fluorescently labelled with antibodies against the epithelial marker E-cadherin (green), the polarisation marker β-catenin (red), the gland mucous cell marker MUC6 (red), the proliferation marker Ki67 (red) or the chief cell marker pepsinogen C (green); nuclei were counterstained with Draq5 (blue). Scale bars: 100 µm; and 25 µm for bottom right panel; for technical reasons smaller spheroids were chosen here compared with the organoids shown in figure 3D. (B) Expression analysis of gastric-specific and epithelial-specific genes from early (P1) and late (P8) passage spheroids, using semi-quantitative RT-PCR. Photomicrographs are presented in reversed colours. PGC, pepsinogen C; EPCAM, epithelial cell adhesion molecule; GADPH, glyceraldehyde 3-phosphate dehydrogenase.