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. 2014 Dec 24;65(2):202–213. doi: 10.1136/gutjnl-2014-307949

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Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

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Human gastric spheroids can be differentiated into organoids. (A) Representative micrographs of different stages of a single spheroid during the differentiation process induced by withdrawal of Wnt3A and R-spondin1. (B) Global overview of differential gene expression upon withdrawal of Wnt3A and R-spondin1 for 5 days as determined by microarray analysis comparing undifferentiated corpus spheroids with differentiated corpus organoids. (C) Expression changes for a selection of gastric-specific and differentiation-specific genes highlighted in the global overview in (B) provided by microarray analysis. For genes with multiple probes, those with the largest difference were chosen. (D) Confocal micrographs of human corpus organoids (cross sections), fluorescently labelled with antibodies against the epithelial marker E-cadherin (green), the polarisation marker β-catenin (red), the pit mucous cell maker MUC5A (red) or the proliferation marker Ki67 (red); nuclei were counterstained with Draq5 (blue). Scale bars: 100 µm.