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. Author manuscript; available in PMC: 2017 Feb 11.
Published in final edited form as: Cell. 2016 Feb 11;164(4):644–655. doi: 10.1016/j.cell.2015.12.039

Figure 1. Elucidation of DSBs in Xrcc4−/−p53−/− NSPCs.

Figure 1

(A) Illustration of N-myc locus and sgRNA target site (vertical black arrowhead) and location and orientation of HTGTS primer (green arrowhead). Cen, centromere; Tel, telomere. E, exon. (B) Circos plot of the mouse genome divided into individual chromosomes showing the genome-wide HTGTS junction pattern of Chr12-sgRNA-1-mediated bait DSBs in Xrcc4−/−p53−/− NSPCs binned into 2.5-Mb regions (black bars); bar height indicates number of translocations per bin on a log scale. 20,000 junctions from four independent experiments are plotted. Red line indicates recurrent translocations between Chr12 bait DSBs (red arrowhead) and an RDC within Lsamp on Chr16; an RDC within Npas3 on Chr12 is denoted by green line. Blue star denotes translocations to sgRNA off-target site. See also Figure S1.