Table 1.
Antibodies used for immunohistochemistry
| Antigen | Clone | Source |
|---|---|---|
| HIF1 | ESEE122 | Abcam (Cambridge, UK) |
| HIF2 | EP109b | NDCLS (University of Oxford, John Radcliffe Hospital, Oxford, UK) |
| CA9 | M75 | BioScience Slovakia s.r.o. (Bratislava, Slovakia) |
| VEGFA | VG-1 | NDCLS |
| TYMP | PGF44 | NDCLS |
| KDR | FLK1(A3) | Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) |
| KDRp34 | 34a | NDCLS |
| FIH | FIH162c/D6 | NDCLS |
| PHD1 | PHD112/G | NDCLS |
| PHD2 | 366G/76 | NDCLS |
| PHD3 | EG188c | NDCLS |
| DLL4 | D4/37 | NDCLS |
| TSP1 | 8A6B-TSP-1 | Leica Microsystems (UK) Ltd. and Novocastra Reagents (Milton Keynes, UK) |
| CXCR4 | MAB 172 | R&D System (Minneapolis, MN, USA) |
| EPHB2 | AF496 | R&D System |
| EPHB3 | R&D AF432 | R&D System |
| EPHB4 | R&D AF446 | R&D System |
| SOD1 | 30F11 | Novocastra |
| BCL2 | 124 | Dako (Cambridgeshire, UK) |
| FOS | Polyclonal 27436 | Abcam |
| EGF | EGF 10 | Abcam |
| EGFR | F4 | Abcam |
| BNIP3 | Ana40 | Sigma-Aldrich Company Ltd. (Dorset, UK) |
| P53 | DO7 | Dako |
| PI3 | Ab 40755 | Abcam |
| SP1 | SP1 polyclonal | Abcam |
| STAT3 | E121-21 | Abcam |
| LON | 20H1 | NDCLS |
| MEF2D | MEF2D polyclonal | Abcam |
| JMY | HMY117A | NDCLS |
| TRAP1 | TR-1A | LabVision (TorsbySjöväg, Värmdö, Sweden) |
| GST | GST3/GST pi | Abcam |
| NCAM | IB6 | Novocastra |
| CHGA | FLEX polyclonal | Dako |
| SYP | 299 | Novocastra |
HIF hypoxia-inducible factor; CA9 carbonic anhydrase 9; VEGFA vascular endothelial growth factor A; TYMP thymidine phosphorylase; KDR vascular endothelial growth factor receptor 2; KDRp34 vascular endothelial growth factor p34; FIH factor-inhibiting HIF; PHD prolyl hydroxylase dehydrogenase; DLL4 Delta-like 4; TSP1 thymidine phosphorylase 1; CXCR4 chemokine (C-X-C motif) receptor 4; EPH ephrin; SOD1 superoxide dismutase 1; BCL2 B cell lymphoma 2; FOS FBJ murine osteosarcoma viral oncogene homolog; EGF epidermal growth factor; EGFR epidermal growth factor receptor; BNIP3 BCL2/adenovirus E1B 19 kDa interacting protein 3; PI3 peptidase inhibitor 3; SP1 Sp1 transcription factor; STAT3 signal transducer and activator of transcription 3; LON Lon protease; MEF2D myocyte enhancer factor 2D; JMY junction-mediating and regulatory protein, p53 cofactor; TRAP1 TNF receptor-associated protein 1; GST glutathione S-transferase; NCAM neural cell adhesion molecule; CHGA chromogranin A; SYP synaptophysin