Figure 1.

Latex Bead Phagosome Motion Inside Dictyostelium Cells and in Cell Extract

(A) Position-time plots of early phagosomes (EPs), late phagosomes (LPs), and endosomes inside Dictyostelium cells. The distance traveled along a curved trajectory (assumed to be a microtubule) was calculated as a function of time. MT orientation inside cells is uncertain.

(B) Position-time plots of purified EPs, LPs, and endosomes moving along single microtubules in an in vitro motility assay. The microtubule orientation is shown on the right.

(C) Velocity of motile EPs, LPs, and endosomes inside Dictyostelium cells and in vitro. Direction of motion (toward plus or minus end of MT) is uncertain inside Dictyostelium cells and has therefore not been assigned. Plus (P)- or minus (M)-directed motion is indicated for in vitro motion. Error bars show SEM. One-way ANOVA shows no statistical difference between these velocities.

(D) In vitro run length of EPs and LPs (35 of each analyzed). Error bars represent SEM.

See also Figures S1 and S2.