Figure S4.

Translation, Proliferation, and Myc Expression Are Reduced in Diapause Embryos, Related to Figure 4

(A) c-Myc protein expression in E4.5 and diapause embryos. Quantification of confocal microscopy images as in Figure 4D.

(B) Expression levels of l-myc in the epiblast of E4.5 and diapause embryos. RNA-seq data based on (Boroviak et al., 2015). Bar graphs show FPKM values and are represented as mean ± SEM. E4.5 epiblast (n = 3); diapause epiblast (n = 2).

(C) Fluorescence staining for Ki67 (red) and DAPI (blue) in E4.5 and diapause embryos. Scale bar, 20 μm.

(D) Quantification of (C). Values are represented as mean ± SD.

(E) EdU incorporation in E4.5 and Diapause embryos. Quantification of confocal microscopy images as in Figure 4F.

(F) OP-Puro incorporation in E4.5 and Diapause embryos. Quantification of Figure 4G.