Figure 3.
Photostimulation of DRN DA Neurons Elicits DA Release in the BNST and CeA
(A) AAV5-DIO-ChR2-eYFP was injected into the DRN of TH::Cre mice to facilitate (B) ChR2 (green) expression in TH+ (red) DRN neurons. White arrows indicate selected co-labeled neurons.
(C) Example of a biocytin-filled, ChR2-expressing, DRN neuron recorded using ex vivo electrophysiology.
(D) ChR2 was activated using eight 5 ms pulses of blue light delivered every 5 s, which elicited a train of action potentials in the ChR2-expressing neuron.
(E) Sagittal brain sections showing dense terminal expression in the BNST and (F) CeA following AAV5-DIO-eYFP injection into the DRN of a TH::Cre mouse.
(G) In vivo FSCV was performed in anesthetized TH::Cre mice following Cre-dependent expression of ChR2 in the DRN. Example color plots and average traces (± SEM) from the BNST (n = 5 mice; 7 recording sites) and (H) CeA (n = 4 mice; 5 recording sites) showing DA release evoked by 150 5 ms pulses of blue light delivered at 30 Hz (left panels) and a representative trace showing eight 5 ms pulses delivered every 5 s (right panels). Eapp, applied potential. Insets show cyclic voltammograms from representative color plots.
See also Figures S2 and S3.