Figure S6.
Effect of Photoinhibition in TH::Cre Mice on Behavioral Measures of Arousal and Anxiety-Related Behavior, Related to Figure 6
(A) The position of the optic fiber tip above the DRN of TH::Cre mice expressing NpHR (orange crosses) or eYFP (gray crosses) used for behavioral analysis.
(B) No significant difference was detected between NpHR- and eYFP-expressing mice in distance traveled in the open field.
(C) In an assay for novelty preference, optical inhibition of DRN DA neurons had no effect on the time spent in the novelty zone in NpHR- (paired t test: t5 = 0.08958, p = 0.9321, n = 6) or (D) eYFP-expressing mice (paired t test: t6 = 0.4595, p = 0.6620; n = 7).
(E) Representative track from a TH::Cre mouse expressing NpHR in DRN DA neurons in the EPM and (F) open field before light stimulation and during delivery of constant yellow light.
(G) There was no significant effect of photoinhibition on time spent in the open arms of the EPM (two-way ANOVA: group × light interaction: F2,38 = 1.009, p = 0.3742, n = 11 NpHR, 10 eYFP) or (H) time spent in the center of the open field (two-way ANOVA: group × light interaction: F2,46 = 2.138, p = 0.1294, n = 13 NpHR, 12 eYFP) in NpHR- relative to eYFP-expressing mice.
(I) In the resident-intruder task optical inhibition did not significantly affect time spent sniffing the juvenile (NpHR: paired t test – t13 = 0.8025, p = 0.4367, n = 14; eYFP: t9 = 1.628, p = 0.1380; n = 10) or (J) time spent rearing (NpHR: t13 = 0.0619, p = 0.9516, n = 14; eYFP: t9 = 1.679, p = 0.1274; n = 10).
Data are represented as mean ± SEM.