Skip to main content
. Author manuscript; available in PMC: 2017 Jan 12.
Published in final edited form as: Biochemistry. 2015 Dec 23;55(1):157–166. doi: 10.1021/acs.biochem.5b01241

Scheme 1. Kinetic Mechanisms of l-DHFR (Blue) and h-DHFR (Red) at 5 °C, pH 9a.

Scheme 1

aUnder saturating substrate concentrations, the cofactor NADPH predominantly binds to DHFR·THF complex during the catalytic turnover instead of the apoenzyme, except during the first turnover (k1 and k−1, green).