Expression of the top genes in macrophages transfected with hY3 RNA and stimulated with immune complexes (IC) comprised of anti-Ro60, Ro60 and hY3 RNA. Each bar represents a transcript of the top 30 up-regulated genes (A) and down-regulated (B) genes in macrophages treated with hY3 and IC. Also shown are plots of the effect of hydroxychloroquine and IRS661, a TLR7 inhibitor, on gene expression of genes that are up-regulated by hY3 and IC treatments, respectively. In addition, shown are expression of genes with a prior link to roles of epigenetic modifications (C). Regarding candidates with a link to epigenetic modifications, there was a trend towards a downregulation of sirtuin 1 (SIRT1), a histone deacetylase.