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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Autoimmun. 2015 Oct 1;67:36–45. doi: 10.1016/j.jaut.2015.09.003

Fig. 4.

Fig. 4

TNF-α protein expression was sensitive to broad inhibition of lysine acetylation while knockdown of histone deacetylase 3 (HDAC3) only affected IL6 and STAT1 expression. Pre-incubation of THP-1 macrophages with curcumin (20 μM), a lysine acetylase inhibitor, attenuated hY3 stimulated increase of TNF-α protein expression (A). shRNA knockdown of histone deacetylase 3 (HDAC3) had no effect on TNF mRNA transcript (qRT-PCR) (B). The hY3 dependent upregulation of STAT1 (p = 0.03) were significantly attenuated, while there was a trend to reduce IL6 (p = 0.09) when levels of HDAC3 were lowered (C). qRT-PCR results are representative of 13 experiments. Bars represent means ± SEM.