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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Cancer Res Clin Oncol. 2015 Sep 18;142(3):549–560. doi: 10.1007/s00432-015-2047-6

Figure 5. CFZ and SAHA act synergistically to induce endoplasmic reticulum and oxidative stress.

Figure 5

Cells were left untreated or treated as indicated with 48 hour IC50 doses. (a). Detection of an ER stress related proteins (ATF4, CHOP, BiP) by immunoblot in total extracts of cells harvested at 24 hours. α-tubulin is shown as a loading control. (b). Relative ROS was measured from cells harvested at 24 hours using the CM-H2DCFDA fluorescence probe. Values are normalized to protein content and displayed relative to the untreated control. (c). Detection of oxidative stress protein (HSP70) by immunoblot in total extracts of cells harvested at 24 hours. α-tubulin is shown as a loading control. BTZ treatments are shown for comparison purposes.