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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Cancer Res Clin Oncol. 2015 Sep 18;142(3):549–560. doi: 10.1007/s00432-015-2047-6

Figure 6. ROS generated with CFZ and SAHA treatment does not significantly contribute to ER stress and cytotoxicity.

Figure 6

Figure 6

Cells were pretreated for 5 hours with vehicle (4% PBS) or concentrations of NAC as indicated. Pretreatment media was removed and cells were either left untreated or treated with CFZ + SAHA 48 hour IC50 doses. Western blot analysis of total cell lysate harvested at 24 hours was used to detect (a) ubiquitinated proteins and (b) oxidative stress, ER stress, and apoptosis related proteins. α-tubulin is shown as a loading control. (c). Cell viability was determined after 48 hours of treatment by direct counting with trypan blue staining. ±SD are shown (n=3).